Coding

Part:BBa_M11319:Design

Designed by: David Peterson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-26)


D-olivose genes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1313
    Illegal NotI site found at 4561
    Illegal NotI site found at 5823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1103
    Illegal BglII site found at 2031
    Illegal BamHI site found at 1578
    Illegal BamHI site found at 2689
    Illegal BamHI site found at 4013
    Illegal BamHI site found at 4360
    Illegal BamHI site found at 6491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 168
    Illegal NgoMIV site found at 963
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 1358
    Illegal NgoMIV site found at 2021
    Illegal NgoMIV site found at 2413
    Illegal AgeI site found at 144
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 2302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4876
    Illegal BsaI site found at 5404
    Illegal BsaI.rc site found at 2536
    Illegal BsaI.rc site found at 4188
    Illegal BsaI.rc site found at 5546
    Illegal SapI site found at 1288
    Illegal SapI.rc site found at 1678


Design Notes

These genes are for streptomyces to use when synthesizing new compounds.


Source

The promoter and ribosome binding sites came from biobrick parts. The sugar genes were converted into biobricks using PCR from the plasmid pLNR. THe glycosyltransferase urdGT2 came from streptomyces genomic DNA.

References